Human TLR4 Stable Cell Line

Name	Human TLR4 Stable Cell Line
Supplier	Novus Biologicals
Catalog	NBP2-26268
Category	Cell Line
Prices	$849.00
Sizes	1 vial
Applications	FA
Gene	TLR4
Species	Human
Growth Mode	Adherent Morphology: Epithelial
Description	The TLR4 stable cell line can be used for TLR4 flow cytometric calibration and detection control as well as TLR4-dependent functional assays. TLR4 expression in this stable cell line has been validated by Western blotting (Fig. 1) and flow cytometry (Fig. 2). Functional activity of this stable cell line has been validated by the NF-kB/SEAPorte™ Assay Kit (NBP2-25286, Fig. 3). Contents: 3~4 x 10^6 cells Biosafety Level: 2
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Product images

Western Blot: Human TLR4 Stable Cell Line [NBP2-26268] - Analysis of TLR4 expression in the TLR4 stable cell line using an HA antibody (20 ug total protein/lane). Legend. Vect: Vector control stable cell line; TLR4: TLR4 stable cell line.

Flow Cytometry: Human TLR4 Stable Cell Line [NBP2-26268] - Analysis of the TLR4 stable cell line. The assay was performed using the NF-kB SEAPorter™ Assay Kit. The Vector control stable cell line and TLR4 stable cell line were co-transfected with NF-kB/SEAP reporter plasmid and MD-2/CD14 expression plasmid for 16 h. Cells were stimulated with LPS for 24 h followed by SEAP assay.

Flow Cytometry: Human TLR4 Stable Cell Line [NBP2-26268] - Flow analysis of TLR4 expression in the TLR4 stable cell line. Cell surface expression of TLR4 in the TLR4 stable cell line was analyzed by flow cytometry using a FITC-conjugated TLR4 antibody and compared with the Vector control stable cell line. Cell Surface TLR Staining Flow Kit was used for this test.